|
KMID : 0878220130250030208
|
|
Journal of Sasang Constitutional Medicine
2013 Volume.25 No. 3 p.208 ~ p.217
|
|
|
Autophagy inducing Effect of modified Yeoldahanso-tang and its related Proteins in SH-SY5Y cells
|
|
Kim Hee-Ju
Bae Na-Young
Jang Moon-Hee
Yang Hyun-Ok
Ahn Taek-Won
|
|
|
Abstract
|
|
|
Objectives: Modified Yeolda-Hanso tang (MYH) is a traditional herbal formula in Korea for various diseases. MYH is containing the 10 herbs : Pueraria lobata (Willd.) Ohwi, Angelica tenuissima Nakai, Scutellaria baicalensis Georgi, Platycodon grandiflorum (Jacq), Angelicae Dahurica, Cimicifuga heracleifolia Kom, Raphanus sativa L., Polygala tenuifolia (Willd), Acorus gramineus Soland and Dimocarpus longan Lour. The 10 herbs is constituted as a ratio of the 6:4:2:1:2:2:2:4:6:6. We investigated neuroprotective effects of MYH on human neuroblastoma SH-SY5Y cells and evaluated the ability of MYH to prevent and treat for neurodegenerative diseases such as Parkinson’s disease via basal autophagy enhancement.
Methods: Pharmacological induction of Autophagy by MYH in SH-SY5Y cells:
Induction of autophagy by MYH in human neuroblastoma SH-SY5Y cells was carreid out by immunoblot analysis with several autophagy markers. SH-SY5Y cells were treated with MYH at the concentration of 400 and 800 ?g/ml for 24 hr. Specifically, the autophagosome proteins LC3 II and Atg5 levels were increased and autophagy pathway related proteins such as beclin-1, PI3 Kinase class III protein, ULK1, mTOR and AMPK were activated.
Conclusions: MYH can enhance the induction of autophagy through key regulator AMPK, mTOR, and Beclin-1 and it should be considered as a possible candidate of neuroprotective agents for such as Parkinson’s disease.
|
|
|
KEYWORD
|
|
|
Modified Yeolda-Hanso tang (MYH), Neuroprotection, Parkinson’s disease, Autophagy
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|